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KMID : 0613820190290091027
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Journal of Life Science
2019 Volume.29 No. 9 p.1027 ~ p.1033
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Osteoblastogenic Activity of Tenebrio molitor Larvae Oil on the MG-63 Osteoblastic Cell
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Seo Min-Chul
Baek Min-Hee
Lee Joon-Ha
Lee Hwa-Jeong
Kim In-Woo
Kim Sun-Young
Hwang Jae-Sam
Kim Mi-Ae
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Abstract
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Recently, Korea has seen a rapid increase in the elderly population. As a result, osteoporosis, a geriatric disease, has become a social problem. To investigate the novel and natural materials for promoting osteoblastogenesis, we investigated the osteoblastogenic activity of Tenebrio molitor larvae oil (TMO) on the MG-63 preosteoblast cells. The cytotoxicity and proliferation effects of TMO on MG-63 cells were measured by MTS assay. There was no cytotoxicity up to 80 μg/ml. At 40 and 80 μg/ml of TMO (treated for 48 hr), cell proliferation was elevated about 120% compared to the control. The osteoblastogenic activity of TMO was measured with alkaline phosphatase (ALP) activity at 5 days. Doses of 5 to 80 μg/ml of TMO increased ALP activities significantly compared with the control. In addition, expression of ALP and Runx2 (osteoblastogenic markers) were markedly increased after treatment of TMO for 5 days. These results provide evidence that TMO promotes osteoblastogenesis by increasing the gene and protein expression of ALP and Runx2, and they suggest that TMO may be a potential agent for bone formation and preventing osteoporosis.
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KEYWORD
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Alkaline phosphatase, bone formation, MG-63, osteoblastogenesis, Tenebrio molitor
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